![]() ![]() It does so by partitioning thousands of cells into nanoliter-scale Gel Bead-In-Emulsions (GEMs) where all generated cDNAs share a common barcode. The 10x GemCode Technology samples a pool of ~750,000 barcodes to separately index the transcriptome of each cell. The technology permits high throughput single cell transcriptomics from a variety of cell types (up to 50 μm in diameter) as well as single-nuclei profiling using 10X Genomics approved protocols. Unveil a new understanding of the adaptive immune response in hundreds to tens of thousands of lymphocytes.The Chromium Single Cell Solution delivers a scalable microfluidic platform for digital gene expression profiling of up to 240,000 cells per run (8 wells). Single Cell Immune Profiling & antigen specificity (using Feature Barcoding technology)ĭetermine antigen specificity of single T cells with Feature Barcode peptide-MHC multimers to study the dynamic interactions between lymphocytes and antigens. Seamlessly combine cell surface protein expression measurements along with immune repertoire and gene expression measurements from the same single cell. Single Cell Immune Profiling & cell-surface protein (using Feature Barcoding technology) The new solution enables assembly of full-length V(D)J sequences on a cell-by-cell basis, providing high resolution insights into the adaptive immune system. Tool for profiling full-length paired V(D)J transcripts from hundreds to tens of thousands of lymphocytes. Identify perturbations of cellular transcripts mediated by hundreds of different CRISPR sgRNAs in one experiment. Gene Expression CRISPR screening (using Feature Barcoding technology) Seamlessly measure both gene- and cell surface protein-expression in the same cell to identify protein isoforms, detect protein for low abundance transcripts, and further increase phenotypic specificity. Gene Expression & cell-surface protein (using Feature Barcoding technology) High-throughput, single cell gene expression measurements that enable discovery of gene expression dynamics and molecular profiling of individual cells and cell types. After sequencing, downstream bioinformatics tools use the cellular barcodes to group transcripts that originated from the same cell, revealing the transcriptome and/or the full-length T- or B-cell receptor sequences of each individual cell. For immune repertoire profiling, the cDNA undergoes targeted enrichment for T- or B-cell receptor transcripts prior to library preparation. The barcoded cDNA is then pooled for downstream processing and library preparation. Single cell suspensions loaded onto the system are partitioned into GEMs, where transcripts are tagged with cell-specific barcodes. The Chromium System also enables single cell transcriptional RNA profiling of hundreds to tens of thousands of individual single cells. After sequencing, the resulting barcoded short read sequences are analyzed with bioinformatics that use the barcode information to map reads back to their original HMW DNA, single cell, or single nucleus of origin. Barcoded products are pooled for downstream reactions to create short-read sequencer compatible libraries. Gel Beads and samples are then added to an oil-surfactant solution to create Gel Beads in EMulsion (GEMs), which act as individual reaction vesicles in which the Gel Beads are dissolved and the sample is barcoded. Gel Beads, containing barcoded oligonucleotides, are mixed with a sample, which can be high molecular weight (HMW) DNA, cells, or nuclei. The 10X Genomics Chromium System, provides a reagent delivery method that enables thousands of micro-reactions in parallel, each containing an identifying barcode such that all sequencing library fragments produced within a partition share a common barcode. Parse Biosciences single cell sequencing.Directional RNA-seq (total RNA, mRNA, miRNA).(Oxidative) Reduced Representation Bisulfite Sequencing. ![]()
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